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KMID : 0381919750050010031
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Korean Journal of Microscopy
1975 Volume.5 No. 1 p.31 ~ p.43
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Studies on Ultrastructure and Virus Infection of Aspergillus ochraseus
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?永健/Deung, Young-Kun
柳英善/李培咸物學科/Lew, Young-Sern/Lee, Bae-Ham
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Abstract
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These studies were carried out to detect the presence of infected virus-.like particles and also were observed the ultrastructures of Aspergillus ochraseus isolated from kokja and Korean ginseng.
The results of ultrastructures of Aspergillus ochraseus are summarized as follows:
1. In fungal cells, nuclei were enclosed by a irregular double membrane and nucleoli in the nucleus.
2. In cytoplasm, mitochondria, rough endoplasmic reticulum with ribosomes and glycogen
were scattering distributed and many lomasomes also observed.
3 The osmiophilic bodies of fungal cells existed in the vesicles.
4 The cell walls were composed of a low electron dense materials.
5. Conidia cell walls were extremely thick and possessed the high electron density of outer
coat.
The virus-like particles were observed in the hyphae of Penicillium chrysogenum Q-176. These virus-like particles measured 350 A in diameter. But strains of Aspergillus ochraseus, showinesome vesicle particles were, also observed obout 800 A in diameter - in the central region of young fungal hyphae.
Based on the results of these experiments, it can not be determined virus particles or
not. The further studies to determination of virus particles will be proceeded by the chemical, physical and biological assay methods.
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